Definition of technical terms for polyclonal antibodies
Chinese name: Polyclonal antibody English name: Polyclonal antibody
Definition 1: Antibodies produced by multiple B cell clones can bind to different antigenic epitopes and have different immunoglobulin categories. Discipline: Immunology (First Level Discipline); Immune system (secondary discipline); Immunological Molecules (Third Level Discipline)
Definition 2: A mixture of immunoglobulins produced for a specific antigen, each immunoglobulin capable of recognizing an epitope on the antigen molecule. Discipline: Biochemistry and Molecular Biology (First Level Discipline); General Introduction (Secondary Discipline)
Definition 3: Mixed antibodies against different antigenic epitopes produced by the body after multiple antigenic epitopes stimulate the immune system. Discipline: Cell Biology (First Level Discipline); Cellular immunity (secondary discipline)
Antigens stimulate the body, producing immunological reactions. A group of immunoglobulin that is synthesized and secreted by plasma cells of the body and has specific binding ability to antigens is called immunoglobulin. This immunoglobulin that has specific binding ability to antigens is called antibody.
An antigen is usually composed of multiple antigenic determinants, and the antibody produced by a B lymphocyte receiving the antigen stimulated by one antigenic determinant is called a monoclonal antibody. Multiple antigenic determinants stimulate the body, resulting in the production of various monoclonal antibodies. These monoclonal antibodies are mixed together to form polyclonal antibodies, and the antibodies produced within the body are called polyclonal antibodies; In addition to the diversity of antigenic determinants, the same type of antigenic determinant can also stimulate the body to produce five types of antibodies, including IgG, IgM, IgA, IgE, and IgD.
The molecular structure on the antigen that can cause the body to produce antibodies is called the antigenic determinant cluster. There can be several different antigenic determinants on an antigen, which leads to the production of several different antibodies in the body, ultimately resulting in plasma cells producing antibodies. A plasma cell population that only acts on one antigenic determinant is a pure line, which is called Clone in English. The specific antibody produced by a clone is called a monoclonal antibody. Monoclonal antibodies can bind specifically to a single antigenic determinant, just like a missile hitting its target with precision. On the other hand, even within the same antigenic determinant cluster, antibodies can be produced by several clones in the body, forming a mixture of several monoclonal antibodies, known as polyclonal antibodies
Polyclonal antibodies, also known as polyclonal antibodies. The corresponding is called monoclonal antibody, abbreviated as mAb.
Generally, it includes the following steps:
1. Prepare antigens.
2. Select experimental animals.
3. Animal immunity.
4. Take blood samples for testing to see if immunization is successful.
5. If successfully immunized, kill the experimental animals and collect all serum.
6. Purify the antibody.
7. Identify antibodies. Including purity and specificity.
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