The accuracy, precision, sensitivity, specificity, stability and other indicators of ELISA Kit determine the quality of the product.

High quality reagent kits require testing for these indicators. At present, high-quality test kits are generally defined to detect eight indicators.

Standard 1: Recovery rate

Evaluate the degree to which the measurement results of the reagent kit tend towards the true value. A high or low recovery rate can produce false positive or false negative results, affecting the accuracy of the measured values.

Standard 2: Natural sample testing

Ensure that the reagent kit can detect natural samples and appropriate sample types. If the detection type of natural samples is not determined, it may lead to false positive or false negative results.

Standard 3: Cross reactivity

Evaluate the specificity of the reagent kit and determine the binding ability of other molecules to antibodies. The greater the cross reactivity, the poorer the specificity of the reagent kit.

Standard 4: Interference Experiment

Determine the impact of other molecules on the interaction between antigens and antibodies. The greater the interference, the less accurate the measured value of the test substance.

Standard 5: Stability Test

Ensure the stability of the reagent kit during long-term storage. The better the stability, the longer the storage time of the reagent kit.

Standard 6: Precision

The indicator that the evaluation kit can obtain similar results when repeatedly measuring the same sample. A reagent kit with poor precision can result in poor reproducibility of the results when testing the same sample repeatedly.

Standard 7: Minimum Detection Limit

Evaluate the sensitivity of the reagent kit, the lower the value, the higher the sensitivity of the reagent kit.

Standard 8: Linear Experiment

Evaluating the accuracy of the reagent kit, whether the results are too high or too low, can affect the accuracy of the test results.

ELISA kits in the market are divided into three categories: low-end, mid-range, and high-end, and are generally distinguished by these eight quality control standards. The reagent kit with only linear experiment detection report belongs to low-end products, the reagent kit that completes precision, minimum detection limit, and recovery rate detection belongs to mid-range products, and the reagent kit that completes cross experiment, interference experiment, stability, natural sample detection and other eight indicators detection belongs to high-end products.

ELISA kit category

What other testing indicators do you pay attention to when choosing ELISA kit products?

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